Sanger sequencing: Common primers provided by the Nevada Genomics Center
All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R.
Please note: there are several primers called: M13F, and M13R. Because of this you should be sure the primer sequence listed below is homologous to your vector’s priming site.
| Name | Sequence |
|---|---|
| M13(-21)F | TGTAAAACGACGGCCAGT |
| M13(-40)R | CAGGAAACAGCTATGAC |
Please check these primer sequences against your vector sequence before you have us use them on your samples!
Please see the guidelines on Sanger Sequencing: Optimal Amount of Template and Primer.